After the plates were incubated and grown, the morphology was observed and recorded and a Gram stain was performed. Gas production was also monitored, looking for fissures produced by production of gas during fermentation. The broth starts out as red but if acid is produced the broth turns yellow. Identification of bacteria is important because they are not only useful but potentially dangerous as well. Plan the length of your informative abstract.
Once these results were discussed with the instructor as incorrect, gram-staining had to be redone. The mixture was soaked with Crystal violet for 30 sec. Example of a Microbiology Unknown Lab Report by Taylor Autry Introduction In this paper I will discuss the processes of how I came to find my two unknown bacteria. Review the lab-report rubric before you start writing. See Table 1 and Flow Chart 1 for results of Bacteria 1 and Table 2 and Flow Chart 2 for results of Bacteria 2. This is achieved by a rotor or impeller, which is driven by an external source of power to move a row of blades so as to. The cells from very thin of bacteria on the surface by swarming, and interspersed with periods and the colony has a distinct zonation or rigns of growth at 37C but the true is they grows best at 25C according to the broth grow.
Introduction: This experiment was centered on metabolic and biochemical testing procedures. No clearing on the side of the streakNegativeGram - Organism does not produce gelatinase. The title says what you did. Plates labeled Mac Light 1 and Mac Creamy 2 Sample plates Berb 3, Light 1 and Creamy 2 were placed in the refrigerator. To verify Escherichia coli is the gram negative bacteria a nitrate test was performed. Centers for Disease Control and Prevention, 17 Jan.
A negative casein test removed S. So I think my unknown was Proteus vulgaris. The first unknown in 123 found to be a gram negative bacteria, was identified as Pseudomonas aeruginosa. In both cases, discussion and conclusion sections help to synthesize the findings by tying them to the objectives and discussing the implications. Explain why you chose 1 interpretation over another. After being extra careful a second isolation was performed, and when the Gram stain was observed there were only Gram-positive rods.
Not all of the tests were performed on every culture, however, as some of the tests were used only for gram + or gram — bacteria, while others were even more specific and used only for cocci bacteria. Daphny Maldonado Bio Lab 2107 Kiah Britton W 10-12:30 Is H20 Bad for You? A gram stain was performed to assess the shape and other characteristics of the bacteria, and to ensure that there was no gram positive contamination. The streak plate that was originally done, showed a heavy green pigment, which is a main characteristic of Pseudomonas auruginosa. Koneman's Color Atlas and Textbook of Diagnostic Microbiology. After these two days, only one distinct colony was present. Gram-staining was utilized as a first step to differentiate between gram-positive and gram-negative.
After a successful Gram stain it was classified as a Gram positive cocci which narrowed it down to three bacteria; Staphylococcus aureus, Staphylococcus epidermidis, and Enterococcus faecalis. It is suggested that culture 11 is a sample of Enterobacter aerogenes. The techniques involved in this study included; what type of agar to use, inoculating, proper sterilization, gram staining and how to handle bacteria in order to avoid contamination. The tube contained two bacterium; one Gram positive + and one Gram negative -. Since the unknown bacteria was a gram negative rod, a gelatin test was performed and the organism was inoculated into a gelatin agar tube and placed into the incubator. Background Information on Bacillus subtilis Bacillus subtilis is a very well-studied microbe.
The nitrate test was used to show if a bacterium would be able to reduce nitrate to nitrite or other nitrogenous compounds. An informative abstract gives the reader a general idea of the research conducted in the lab report. The gram positive bacteria was inoculated into a mannitol fermentation tube and placed into the incubator. The method results should be in a table format see examples below. Next a Simmons citrate test was performed to determine if the organism can use citrate as its sole carbon source. Be as specific as possible, and do not describe scientific phenomena unrelated to microbiological science.
Also knowledge of different bacteria helped others make antibiotics used today. The first step that should be taken very seriously is cook meat well, especially ground beef. Once it was determined that it was a rod-shaped bacterium, only two possible options were left: Bacillus subtilis and Bacillus cereus. For this test, I was able to finalize the result of my unknown to be Proteus vulgaris. Some problems encountered on this activity is the handling and contamination. After the Gram stain was completed, the bacteria were streaked on a Mannitol-Salt Agar plate and a Catalase test was performed. After observation, the H2S test had a negative result, which pointed to Escherichia coli.