Directly following the solubility test, the conductivity test was performed. The only difference between the two tests was that in the pH test, a pH probe was connected to the LoggerPro system instead of a conductivity probe. The Chromic Anhydride test caused Aldehydes to turn blue, and Ketones orange. There was no change to the colorless solution due to Ketones being less susceptible to oxidation. Bacteria that survive and employ the citrate begin the process by converting ammonium phosphate to ammonia and ammonium hydroxide. The Iodoform reaction would have a positive result if there is a pale yellow precipitate. Proper disposal of compounds is very important because they can potentially have harmful effects on their surroundings.
If a graph is plotted with the time period as ordinate and the distance of the point of suspension from C. P , Boiling Point B. If Simmons slant changes color to blue then it is positive for citrase; otherwise, no visible change is a negative result. We balanced the bar on a sharp wedge and marked the position of its C. P and Infrared Spectroscopy Methods and Background The main objective of this lab is to identify the given unknown organic compounds with various methods. Waste Management: All chemicals, unless you were told to do so, were asked to be dispensed in separate containers.
The index of hydrogen deficiency determines the saturation of a compound double bonds, triple bonds or rings. In order to dispose of it correctly, the substance and its physical and chemical properties had to be identified. There were five possibilities of Gram positive + bacteria; Bacillus cereus, Bacillus subtitis, Staphylococcus aureus, Straphylococcus epidermidis, andEnterococcus faecalis. The bacterium that produces greenish discoloration of the medium around the streak indicates alpha hemolysis. From the results of these initial tests and the given list… 1033 Words 5 Pages mentioned that Catherine is a keen observer and through this ability she notices a lot of things about the Company. If the bacterium is able to ferment sugar, the broth color will change from pink to yellow indicating positive results whereas no color change is negative4for carbohydrate fermentation.
We displaced the bar slightly to one side of the equilibrium position and let it oscillate with the amplitude not exceeding 5 degrees. If there is no visible change in the medium, it is negative or gamma hemolysis. Successor of Basic Chemistry Lab-Include-Acid-base titrations, alkali solutions, Amperometry, Colourchange, Complexometric, titrations, Conductance, equivalence point, Henderson-Hasselbalch equation, History, indicator error, indicators, isothermal titration, pH curve, pH indicator, pH meter, potentiometer, Precipitation, Redox titrations, Spectroscopy, Thermometric titrimetry, titrant, Titration, Titration curve, titration methods, titrator, Volumetric analysis ,zeta potential titration Objective: In this experiment, you will identify an unknown, white compound by comparing the results of careful observations and tests that you make on the unknown to observations and tests made on a series of standard compounds. An excess of ammonia should be avoided. This carbon atom is more likely to undergo a nucleophillic attack, especially if the oxygen is protonated.
However, the Gram stain technique had already provided the elimination of Gram positive + bacillus as a result of observation under light microbe scope. The experiment In today's experiment, you and the members of your group will perform the same tests that Jason has to carry out in order to solve his dilemma. Small Aldehydes and Ketones are easily dissolved in water but as the chain increases in length, its solubility decreases. A precipitation reaction is a reaction in which an insoluble solid compound is formed when solutions of two soluble compounds are mixed. Each one of these compounds has a different purpose.
According to the theorem of parallel axes, if I G is the moment of inertia of the pendulum about an axis through C. This seemly simple task is, however, can be quite complex, as there are many ways by which one can identify a metal, each with varying amounts of accuracy. The Glucose fermentation result was positive which eliminated Pseudomonas aeruginosa. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Iodoform Reaction A positive result of this test would be indicated by a pale yellow precipitate in the test tube. If the medium does not change colors after the addition of the zinc powder then the test is positive for nitrate reduction. During the first part of the experiment, the unknown compound was narrowed down to 4 compounds, all of which were sulfates, but it was suspected to be either potassium sulfate or sodium sulfate.
Because it moves as one rigid body and we take into account the moment of inertia of the whole body. Reactivity of this group is ruled by the electron imbalance in the πorbitals of the bond between a more electronegative and a carbon atom. However, if no bubble is present, the culture is negative for catalase and result was recorded. Iodine-potassium reagent was then added drop wise until a faint color of iodine persists. To determine a basic physical property of the compound, a solubility test was performed in a test tube by mixing a 0.
Some common household lipids are butter, canola oil, and extra virgin olive oil. The Bunsen burner was connected to the gas valve using a rubber hose and it was then lit and adjusted to have a steady flame. Struble, 2009 The Proteus species have an extracytoplasmic outer membrane. Many of the chemical and physical properties of the unknown compound were discovered and recorded. .
Girls were told to wear ponytails. Record the code number of your test tube in your lab notebook. Neither you, nor the coeditors you shared it with will be able to recover it again. This was added slowly, together with shaking a dilute solution of ammonia until the precipitate just dissolved. To determine the presence of catalase, drops of hydrogen peroxide were placed onto a clean microscope slide, and Gram + bacteria was transferred to the hydrogen peroxide puddle using a wood stick.